In 2009, PacBio published a paper expanding the principle into a full-blown sequencing technique. Once again, each nucleotide was labeled with a different colored fluorophore detectable by the ZMW to determine which base had been incorporated. The fluorophores were attached such that they would be cleaved off during the chemical reaction incorporating the base into the growing DNA strand; they would then diffuse out of the ZMW so that the next fluorophore could be detected. Sequencing took place on a chip with many wells simultaneously — a different type of parallelization where each well detected a single DNA molecule undergoing the same basic chemical reaction.
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2025年,全国省际贸易销售额占全部销售额的比重升至41%,跨省跨区交易电量占全国电力市场交易电量的比重升至24%。社会物流总费用与GDP的比率降至13.9%,创有统计以来的最好水平。这“两升一降”,反映要素实现更大范围优化配置,市场交易成本不断降低。